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We investigated the isolation method of mushroom infesting pests, Lycoriella mali, Coboldia fuscipss, Histiostoma sp. from mushroom-growing compost. Sugar solution of different densities (0, 10, 20, 30, 40, 50%) was tested to provide a means of separating mushroom pests from the compost media. Thus, 40% sugar solution was suitable for isolation. The sieve size to entrap the pests was 30∼140 mesh; Lycoriella mali was trapped mainly 30∼65 mesh sieve, Coboldia fuscipes was caught mainly 30∼100 mesh sieve, Histiostoma sp. was trapped mainly 65∼140 mesh sieve. An isolation procedure was as follows; The infested compost was disintegrated in water and poured onto a set of 16, 30, 80, 140-mesh sieve. The material caught in the sieve is added in 40% sugar solution and then most compost particle were massed at the bottom while the supernatant contains mushroom pests. The upperlayer material was poured into a Seperatory funnel and the sediment at the bottom is drained off. The remaining material are washed off examination dish for study.
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